Table 1: [Summary of competitive, non-competitive and uncompetitive inhibition models.]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

Assay Guidance Manual [Internet].

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Table 1:

Summary of competitive, non-competitive and uncompetitive inhibition models.

Inhibition	Description	K_i	K_i’	K_i’/K_i
Competitive	The inhibitor binds only to free enzyme. This binding most often occurs in the active site at the precise location where substrate or cofactor (being evaluated in the MOA study) also binds.	finite	Infinite	infinite
Mixed	These inhibitors display properties of both competitive and noncompetitive inhibition.	finite	Finite	> 1
Noncompetitive	The inhibitor binds equally well to both free enzyme and the enzyme-substrate complex. Consequently, these binding events occur outside the active site.	finite	Finite	= 1
Uncompetitive	The inhibitor binds only to the enzyme-substrate complex at a location outside the active site.	infinite	Finite	= 0

From: Mechanism of Action Assays for Enzymes

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Strelow J, Dewe W, Iversen PW, et al. Mechanism of Action Assays for Enzymes. 2012 May 1 [Updated 2012 Oct 1]. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Table 1: [Summary of competitive, non-competitive and uncompetitive inhibition models.].
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