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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Assay Guidance Manual [Internet].

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Figure 8 . – Comparison on enzyme data for a system with a proper slope of 1 and another displaying a sigmoidal relationship (ex.

Figure 8

– Comparison on enzyme data for a system with a proper slope of 1 and another displaying a sigmoidal relationship (ex. slope of 2) between the substrate concentration tested and the rate observed.

From: Mechanism of Action Assays for Enzymes

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