Figure 5. [siRNA optimization plate layout examples...]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

Assay Guidance Manual [Internet].

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Figure 5.

siRNA optimization plate layout examples. (A) has a wider range of transfection reagent concentrations to judge, given a fixed siRNA concentration (the edge wells are intentionally left blank); while in (B) the concentrations of siRNA and transfection reagent are optimized together. Different cell seeding density can also be tested along with the two factors in different plates. NC-negative control; PC-positive control; TRx.R – transfection reagent. In (B) each of the four blocks (4x4) in the center is a factorial design of siRNA and transfection reagent concentrations

From: Cell-Based RNAi Assay Development for HTS

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Martin S, Buehler G, Ang KL, et al. Cell-Based RNAi Assay Development for HTS. 2012 May 1 [Updated 2013 May 1]. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Figure 5. [siRNA optimization plate layout examples...].
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