Figure 10. . Screening a large library of non-pooled siRNAs enables determination of biased seed sequences (20).

Figure 10.

Screening a large library of non-pooled siRNAs enables determination of biased seed sequences (20). Two siRNAs targeting SCAMP5 appear to significantly down-regulate the assay response. However, siRNAs containing the same hexamer sequences exhibit a clear bias towards down-regulating the assay. Therefore, the SCAMP5 siRNAs would be flagged as highly suspicious and are most likely OTEs.

From: Cell-Based RNAi Assay Development for HTS

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Assay Guidance Manual [Internet].
Markossian S, Grossman A, Arkin M, et al., editors.
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