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Papagiakoumou E, editor. All-Optical Methods to Study Neuronal Function [Internet]. New York: Humana; 2023. doi: 10.1007/978-1-0716-2764-8_10

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All-Optical Methods to Study Neuronal Function [Internet].

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A multipart image shows the axicon based Bessel module. Part a and b represents Gaussian and Bessel focus respectively. Part c and d has the changes in the displacement and fluorescence with respect to time in the x-axis.

Fig. 9

Bessel focus scanning is resistant to axial motion artifacts. (a, b) Images obtained with 2D scans of a Gaussian focus and a Bessel focus, respectively, from an awake Thy1-YFP line H mouse. (c, d) Brain motion (upper panel, quantified as the lateral image displacement with time) causes (c) large changes of fluorescence signal from two YFP+ dendrites (ROI1 and ROI2) in Gaussian focus scanning mode, (d) but not Bessel focus scanning mode. (From Ref. [15])

From: Chapter 10, High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy

Copyright 2023, The Author(s)

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