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Langerak AW, editor. Immunogenetics: Methods and Protocols [Internet]. New York: Humana; 2022. doi: 10.1007/978-1-0716-2115-8_19

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Immunogenetics: Methods and Protocols [Internet].

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Fig. 2. A schematic of dT-primed first-strand cDNA synthesis followed by two rounds of successive PCR for amplification of cDNA sequences.

Fig. 2

A schematic of dT-primed first-strand cDNA synthesis followed by two rounds of successive PCR for amplification of cDNA sequences. After post-PCR purification, size selection, and quality analysis, the library is ready for sequencing

From: Chapter 19, Bulk Sequencing from mRNA with UMI for Evaluation of B-Cell Isotype and Clonal Evolution: A Method by the AIRR Community

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