FIGURE 27.3.. (Top left) Zebrafish (Danio rerio).

FIGURE 27.3.

(Top left) Zebrafish (Danio rerio). (Photograph from the Zebrafish Information Network [ZFIN], © University of Oregon; see also Sprague J, et al. 2006. Nucl Acids Res 34: D581–D585.) (Top right) Adult mouse (Mus musculus). (Middle) Gene editing using CRISPR/Cas9. A gene-specific guide RNA with a trans-activating CRISPR-targeting guide RNA (crRNA) that recruits the Cas9 nuclease for sequence-specific cleavage of genomic DNA. NGG, a protospacer-adjacent motif (PAM), must be located just 3′ of the genomic DNA target sequence. (Reprinted from System Biosciences Inc.; https://www.systembio.com/crispr-cas9-plasmids.) (Bottom) Gene editing using a transcription activator–like (TALE) nuclease (TALEN). TALEN vectors encode proteins designed to specifically bind genomic DNA flanking the desired cleavage site linked to the Fok1 nuclease that catalyzes the site-specific cleavage. (Reprinted from Ramalingam S. 2013. Genome Biol 14: 107–110.)

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From: Chapter 27, Deuterostomes

Cover of Essentials of Glycobiology
Essentials of Glycobiology [Internet]. 4th edition.
Varki A, Cummings RD, Esko JD, et al., editors.
Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022.
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