FIGURE 27.3.. (Top left) Zebrafish (Danio rerio).

FIGURE 27.3.

(Top left) Zebrafish (Danio rerio). (Photograph from the Zebrafish Information Network [ZFIN], © University of Oregon; see also Sprague J, et al. 2006. Nucl Acids Res 34: D581–D585.) (Top right) Adult mouse (Mus musculus). (Middle) Gene editing using CRISPR/Cas9. A gene-specific guide RNA with a trans-activating CRISPR-targeting guide RNA (crRNA) that recruits the Cas9 nuclease for sequence-specific cleavage of genomic DNA. NGG, a protospacer-adjacent motif (PAM), must be located just 3′ of the genomic DNA target sequence. (Reprinted from System Biosciences Inc.; (Bottom) Gene editing using a transcription activator–like (TALE) nuclease (TALEN). TALEN vectors encode proteins designed to specifically bind genomic DNA flanking the desired cleavage site linked to the Fok1 nuclease that catalyzes the site-specific cleavage. (Reprinted from Ramalingam S. 2013. Genome Biol 14: 107–110.)

Download Teaching Slide (PPTX, 11M)

From: Chapter 27, Deuterostomes

Cover of Essentials of Glycobiology
Essentials of Glycobiology [Internet]. 4th edition.
Varki A, Cummings RD, Esko JD, et al., editors.
Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022.
Copyright © 2022 The Consortium of Glycobiology Editors, La Jolla, California; published by Cold Spring Harbor Laboratory Press; doi:10.1101/glycobiology.4e.27. All rights reserved.

The content of this book is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported license. To view the terms and conditions of this license, visit

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.