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Maiato H, editor. Cytoskeleton Dynamics: Methods and Protocols [Internet]. New York (NY): Humana Press; 2020. doi: 10.1007/978-1-0716-0219-5_17

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Cytoskeleton Dynamics: Methods and Protocols [Internet].

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Fig. 3. Mouth pipette production and assembly for aspiration of mouse oocytes.

Fig. 3

Mouth pipette production and assembly for aspiration of mouse oocytes. (a) A fully assembled mouth pipette containing mouth piece-fitted tubing and a pulled glass micropipette separated with a 0.22 μm filter. (b) The process of cleaning isolated oocytes from debris by washing through nine droplets of M2 + dbcAMP. (c) Production of glass micropipettes for mouth pipetting using fire-assisted manual pulling techniques. Micropipettes with sharp or broken edges should be discarded to avoid oocyte death during mouth pipetting

From: Chapter 17, Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes

Copyright 2020, The Author(s)

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