Figure 8. [An example of an agonist...]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Figure 8.

An example of an agonist concentration-response curve in G_αs-coupled receptors. Panel A. The agonist response is plotted as the HTRF ratio (Em665/Em615×10⁴) along with the cAMP standard curve. Note that the agonist produces signals that are within the linear portion of the standard curve. Panel B. The agonist response is plotted as the cAMP level to determine the EC₅₀ values. Panel C. The same agonist response is plotted as % stimulation. Data are analyzed using a four-parameter logistic regression.

From: Measurement of cAMP for G_αs- and G_αi Protein-Coupled Receptors (GPCRs)

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Wang T, Li Z, Cvijic ME, et al. Measurement of cAMP for Gαs- and Gαi Protein-Coupled Receptors (GPCRs) 2017 Nov 20. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Figure 8. [An example of an agonist...].
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