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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Assay Guidance Manual [Internet].

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Figure 5. . Examples of (A) forskolin concentration response-curves at various cell densities for a Gαi-coupled receptor, (B) an agonist concentration-response assayed at 3 μM forskolin and 1,500 cells/well as selected from Panel A, and (C) the agonist concentration-response from Panel B expressed in cAMP level.

Figure 5.

Examples of (A) forskolin concentration response-curves at various cell densities for a Gαi-coupled receptor, (B) an agonist concentration-response assayed at 3 μM forskolin and 1,500 cells/well as selected from Panel A, and (C) the agonist concentration-response from Panel B expressed in cAMP level.

From: Measurement of cAMP for Gαs- and Gαi Protein-Coupled Receptors (GPCRs)

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