Figure 10. [An example of antagonist concentration-response...]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Figure 10.

An example of antagonist concentration-response curves in G_αs-coupled receptors. Panel A. The antagonist response is plotted as the HTRF ratio (Em665/Em615×10⁴) along with the cAMP standard curve. Note that the antagonist produces signals that are within the linear portion of the standard curve. Panel B. The antagonist response is plotted as the cAMP level to determine the IC₅₀ values, respectively. Panel C. The same antagonist response is plotted as % Inhibition. Data are analyzed using four-parameter logistic regression.

From: Measurement of cAMP for G_αs- and G_αi Protein-Coupled Receptors (GPCRs)

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Wang T, Li Z, Cvijic ME, et al. Measurement of cAMP for Gαs- and Gαi Protein-Coupled Receptors (GPCRs) 2017 Nov 20. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Figure 10. [An example of antagonist concentration-response...].
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