Figure 5: . Principle of protein fragment complementation.

Figure 5:

Principle of protein fragment complementation. (A) Two interacting proteins (blue) are fused to N- and C-terminal fragments of a reporter protein such as GFP or luciferase (gray). Following interaction, the reporter is reconstituted (green). (B) A small molecule (red star) can affect the reporter signal in several ways. The molecule might bind to and specifically inhibit the PPI under interrogation, a ‘true positive’ (top). Alternatively, false positive compounds could interfere with reconstitution of the reporter protein (middle) or block the catalytic activity of the reporter (bottom). Secondary screens are required to identify such false positive events.

From: Inhibition of Protein-Protein Interactions: Cell-Based Assays

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Markossian S, Grossman A, Arkin M, et al., editors.
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