NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.
Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-.
Table 2Stepwise protocol of the PGE2 HTRF assay using LNCaP cells in 384-well format
| Sequence | Parameter | Value | Description |
|---|---|---|---|
| 1 | Reagent | 9.9 μL | 5,000 cells/well (A549, LNCaP, or media) |
| 2 | Compound | 100 nL | Compound addition through acoustic liquid dispenser (final concentration ranges 41.9 nM t0 100 μM) |
| 3 | Time | 16 hr | Upon mixing, incubation at 37 ºC, 5% CO2 |
| 4 | Reagent | 5 μL | PGE2-d2 |
| 5 | Reagent | 5 μL | anti-PGE2-cryptate |
| 6 | Time | 15 sec | Centrifuged at 1,500 RPM |
| 7 | Time | 5 hr | Incubation at room temperature, protected from light |
| 8 | Detector | HTRF | Envision (320 nm, 615 and 665 nm emission |
- Review Potent and selective inhibitors of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (HPGD).[Probe Reports from the NIH Mol...]Review Potent and selective inhibitors of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (HPGD).Jadhav A, Niesen FH, Schultz L, Oppermann U, Maloney DJ, Simeonov A. Probe Reports from the NIH Molecular Libraries Program. 2010
- Structure-activity relationship studies and biological characterization of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase inhibitors.[Bioorg Med Chem Lett. 2014]Structure-activity relationship studies and biological characterization of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase inhibitors.Duveau DY, Yasgar A, Wang Y, Hu X, Kouznetsova J, Brimacombe KR, Jadhav A, Simeonov A, Thomas CJ, Maloney DJ. Bioorg Med Chem Lett. 2014 Jan 15; 24(2):630-5. Epub 2013 Dec 4.
- miR-620 promotes tumor radioresistance by targeting 15-hydroxyprostaglandin dehydrogenase (HPGD).[Oncotarget. 2015]miR-620 promotes tumor radioresistance by targeting 15-hydroxyprostaglandin dehydrogenase (HPGD).Huang X, Taeb S, Jahangiri S, Korpela E, Cadonic I, Yu N, Krylov SN, Fokas E, Boutros PC, Liu SK. Oncotarget. 2015 Sep 8; 6(26):22439-51.
- Understanding human 15-hydroxyprostaglandin dehydrogenase binding with NAD+ and PGE2 by homology modeling, docking and molecular dynamics simulation.[Bioorg Med Chem. 2005]Understanding human 15-hydroxyprostaglandin dehydrogenase binding with NAD+ and PGE2 by homology modeling, docking and molecular dynamics simulation.Hamza A, Cho H, Tai HH, Zhan CG. Bioorg Med Chem. 2005 Jul 15; 13(14):4544-51.
- Review Close teamwork between Nrf2 and peroxiredoxins 1 and 6 for the regulation of prostaglandin D2 and E2 production in macrophages in acute inflammation.[Free Radic Biol Med. 2015]Review Close teamwork between Nrf2 and peroxiredoxins 1 and 6 for the regulation of prostaglandin D2 and E2 production in macrophages in acute inflammation.Ishii T. Free Radic Biol Med. 2015 Nov; 88(Pt B):189-198. Epub 2015 May 9.
- Table 2, Stepwise protocol of the PGE2 HTRF assay using LNCaP cells in 384-well ...Table 2, Stepwise protocol of the PGE2 HTRF assay using LNCaP cells in 384-well format - Probe Reports from the NIH Molecular Libraries Program
Your browsing activity is empty.
Activity recording is turned off.
See more...