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Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-.
Figure 3
A) SDS-PAGE run under non-reducing conditions gives a single band for PKM2 in control treated cells, but diamide treated cells two bands for PKM2. When both conditions are run under reducing SDS-PAGE conditions, a single band for PKM2 is observed. B) This faster migrating PKM2 species is observed in WT PKM2 expressing cells as well as a variety of mutants, except in the case of the C358S mutant PKM2 line. C) Cells were lysed under denaturing conditions, reduced cysteines were blocked with maleimide then oxidized cysteines were reduced and subsequent labeling of those reduced thiols with biotinylated maleimide allowed for streptavidin pull-down.
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- Figure 3, [A) SDS-PAGE run under non-reducing...]. - Probe Reports from the NIH ...Figure 3, [A) SDS-PAGE run under non-reducing...]. - Probe Reports from the NIH Molecular Libraries Program
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