Figure 18: [A549 cells (500/well) were plated...]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

Assay Guidance Manual [Internet].

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Figure 18:

A549 cells (500/well) were plated in 40µL medium containing 2x RealTime-Glo™ reagents. A thapsigargin titration was prepared in medium at 2X concentrations and added to the plate at an equal volume. The final concentrations of thapsigargin ranged from 500nM - 0.5nM. The vehicle control was 0.1% DMSO. Luminescence was monitored every hour for 72 hours using a Tecan Infinite^® 200 Multimode Reader with Gas Control Module (37°C and 5% CO₂).

From: Cell Viability Assays

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Riss TL, Moravec RA, Niles AL, et al. Cell Viability Assays. 2013 May 1 [Updated 2016 Jul 1]. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Figure 18: [A549 cells (500/well) were plated...].
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