Figure 7. Characterization of CID 3528206 (SID 96099781) as a TORC1 specific inhibitor.

Figure 7Characterization of CID 3528206 (SID 96099781) as a TORC1 specific inhibitor

A) Dose response analysis of CID 3528206 (SID 96099781) on five GFP clones. B) Structure of CID 3528206 (SID 96099781). C) TORC1 bypass cell growth assay on CID 3528206 (SID 96099781. 1:10 fold serial dilution of TORC1 bypass cells and BY4741 cells were spotted onto YPD plate supplemented with DMSO, 0.22μM and 30μM CID 3528206 (SID 96099781). The plate was incubated for 48–72 hrs. D) Sch9p phosphorylation assay on CID 3528206 (SID 96099781) to measure the inhibition on TORC1 activity. E) YPK1 phosphorylation assay on CID 3528206 (SID 96099781) to measure the inhibition on TORC2 activity.

From: Profiling a Selective Probe for RTG Branch of Yeast TORC1 Signaling Pathway

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