Figure 11. Sch9 phosphorylation assay on active compounds.

Figure 11Sch9 phosphorylation assay on active compounds

A) Western blot showing the phosphorylated Sch9p (top band) in yeast cells treated with compounds at different concentrations (0.1–100μM). B) The intensity of top band was quantified to show the IC50 of the compounds, designated here by SID.

From: Profiling a Selective Probe for RTG Branch of Yeast TORC1 Signaling Pathway

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Probe Reports from the NIH Molecular Libraries Program [Internet].

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