[Table], Table 1. Strengths and weaknesses of fluorophores commonly used in smFISH. - WormBook

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WormBook: The Online Review of C. elegans Biology [Internet]. Pasadena (CA): WormBook; 2005-2018.

WormBook: The Online Review of C. elegans Biology [Internet].

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Table 1. Strengths and weaknesses of fluorophores commonly used in smFISH.

	Peak Excitation/Emission Wavelength	Photostability	Autofluorescence level	RFP compatible	GFP compatible
Cy5	649/670 nm	Low	Low	Yes	Yes
Alexa 594	590/617 nm	High	Medium	Affected	Yes
TMR	564/570 nm	High	High	No	Yes

From: Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos

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Ji N, van Oudenaarden A. Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos. In: WormBook: The Online Review of C. elegans Biology [Internet]. Pasadena (CA): WormBook; 2005-2018. [Table], Table 1. Strengths and weaknesses of fluorophores commonly used in smFISH.

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