Table 2: [Extraction Solvents]. - Assay Guidance Manual - NCBI Bookshelf

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Table 2:

Extraction Solvents

Extraction Solvent	Solvent Strength	Polarity	BP	Dielectric Constant	% of Solvent in Water	% of Water in Solvent
Heptane	0.01	0	98	1.92	0.0003	0.01
Toluene	0.29	2.4	110	2.4	0.67	0.046
MTBE	0.47	2.5	55	4	4.8	1.5
Diethyl ether	0.38	2.8	35	4.3	6.89	1.3
Methylene Chloride	0.42	3.1	40	9.08	1.6	0.17
Chloroform	0.4	4.1	61	4.81	0.815	0.072
Ethyl Acetate	0.58	4.4	77	6.02	8.7	9.8
Butanol	0.7	3.9	117	17.8	7.8	20.1

From: HPLC-MS/MS for Hit Generation

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Thibodeaux SJ, Yurek DA, McGee JE. HPLC-MS/MS for Hit Generation. [Updated 2017 Jul 8]. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Table 2: [Extraction Solvents].
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