Figure 25: . Reference compound inhibitor IK202 dose response 3 day experimental variability.

Figure 25:

Reference compound inhibitor IK202 dose response 3 day experimental variability. HeLa cells seeded at 5,000 cells/well overnight and treated in dose response with reference compound inhibitors IK202 for 15 minutes followed by stimulation with 25ηg/ml TNF-α for ~35 minutes. Cells were then fixed and stained to measure NF-κB translocation. Plates were analyzed on HCS imager to determine NF-κB translocation using CytoNuc Difference calculation; data was normalized to control and plotted in GraphPad Prism using non-linear regression 3-parameter fit.

From: Nuclear Factor Kappa B (NF-κB) Translocation Assay Development and Validation for High Content Screening

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Assay Guidance Manual [Internet].
Markossian S, Grossman A, Arkin M, et al., editors.
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