nested PCR - Books

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pcr in Paulinella chromatophora Paulinella micropora Cucumis sativus All 4 Gene records

Results: 1 to 20 of 1074 books (6206 items)

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Select item 14589841.

GeneReviews® [Internet].

Adam MP, Feldman J, Mirzaa GM, et al., editors.

Seattle (WA): University of Washington, Seattle; 1993-2024.

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Select item 44036682.

StatPearls [Internet].

Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Select item 26591823.

Database of Abstracts of Reviews of Effects (DARE): Quality-assessed Reviews [Internet].

York (UK): Centre for Reviews and Dissemination (UK); 1995-.

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Select item 16686904.

Holland-Frei Cancer Medicine. 6th edition.

Kufe DW, Pollock RE, Weichselbaum RR, et al., editors.

Hamilton (ON): BC Decker; 2003.

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Select item 40947245.

BioWatch PCR Assays: Building Confidence, Ensuring Reliability: Abbreviated Version.

Committee on PCR Standards for the BioWatch Program; Board on Life Sciences; Division on Earth and Life Studies; Board on Health Sciences Policy; Institute of Medicine; National Research Council.

Washington (DC): National Academies Press (US); 2015 Jun 26.

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Select item 14152806.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 28215247.

PDQ Cancer Information Summaries [Internet].

Bethesda (MD): National Cancer Institute (US); 2002-.

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Select item 41296388.

Human Papillomaviruses.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 2007. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 90.)

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Select item 54056589.

Immunogenetics: Methods and Protocols [Internet].

Langerak AW, editor.

New York: Humana; 2022.

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Select item 431748810.

DNA Mismatch Repair Deficiency Tumour Testing for Patients With Colorectal Cancer: A Health Technology Assessment [Internet].

Assasi N, Blackhouse G, Campbell K, et al.

Ottawa (ON): Canadian Agency for Drugs and Technologies in Health; 2016 Aug. (CADTH Optimal Use Report, No. 5.3b.)

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Select item 406499611.

Rapid detection of health-care-associated bloodstream infection in critical care using multipathogen real-time polymerase chain reaction technology: a diagnostic accuracy study and systematic review.

Warhurst G, Dunn G, Chadwick P, et al.

Southampton (UK): NIHR Journals Library; 2015 May. (Health Technology Assessment, No. 19.35.)

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Select item 236397412.

Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors.

Cambridge: Cambridge University Press; 2007.

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Select item 158168613.

Medical Microbiology. 4th edition.

Baron S, editor.

Galveston (TX): University of Texas Medical Branch at Galveston; 1996.

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Select item 438190014.

Human Papillomaviruses.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 1995. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 64.)

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Select item 333779615.

Can rapid integrated polymerase chain reaction-based diagnostics for gastrointestinal pathogens improve routine hospital infection control practice? A diagnostic study.

Pankhurst L, Macfarlane-Smith L, Buchanan J, et al.

Southampton (UK): NIHR Journals Library; 2014 Aug. (Health Technology Assessment, No. 18.53.)

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Select item 186024216.

WormBook: The Online Review of C. elegans Biology [Internet].

Pasadena (CA): WormBook; 2005-2018.

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Select item 332687217.

Randomised controlled trial and health economic evaluation of the impact of diagnostic testing for influenza, respiratory syncytial virus and Streptococcus pneumoniae infection on the management of acute admissions in the elderly and high-risk 18- to 64-year-olds.

Nicholson KG, Abrams KR, Batham S, et al.

Southampton (UK): NIHR Journals Library; 2014 May. (Health Technology Assessment, No. 18.36.)

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Select item 407316018.

Malaria and Some Polyomaviruses (SV40, BK, JC, and Merkel Cell Viruses).

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 2013. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 104.)

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Select item 150279219.

Molecular Imaging and Contrast Agent Database (MICAD) [Internet].

Bethesda (MD): National Center for Biotechnology Information (US); 2004-2013.

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Select item 420867020.

Guidelines for Stopping Mass Drug Administration and Verifying Elimination of Human Onchocerciasis: Criteria and Procedures.

Geneva: World Health Organization; 2016.

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Supplemental Content

Images search in Bookshelf

FIGURE A12-8Products from nested PCR reactions using universal fdhF primers followed by Amitermes-Gnathamitermes-Rhychotermes clade specific primers on gut templates

NEB 2 log ladder was used. See Figure A12-5 legend for template designations. Slight band in lane Zn was observed inconsistently and infrequently and is attributed to contamination during nested PCR.

Contributed Manuscripts.

Institute of Medicine (US) Forum on Microbial Threats. The Social Biology of Microbial Communities: Workshop Summary. Washington (DC): National Academies Press (US); 2012.
Fig. 2

Workflow of an exon skipping evaluation experiment. RNA evaluation: a 6-well plate is seeded and transfected with two different AON concentrations. Cells are collected after 48 h for RNA extraction, reverse transcription (RT), and nested PCR analysis by densitometry. Dystrophin protein quantification: a 96-well plate is seeded, transfected, and allowed to differentiate for a week. Plates are fixed before being analyzed by myoblot . Created with Biorender.com

Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy.

Arechavala-Gomeza V, Garanto A, editors. Antisense RNA Design, Delivery, and Analysis [Internet]. New York: Humana; 2022.
Figure 5Schematic of primers and amplicons in the poison primer method

Two rounds of nested PCR amplifications are used in which one or two poison primers are added to the first-round PCR mix (top schematic). In the first round reaction, full-length and poison amplicons are produced (middle schematic). During the second round of amplification in which the poison primers are not added, the wild-type and deletion amplicons are produced (lower schematic). Addition of the poison primers in the first round reaction reduces the amount of full-length amplicon produced, thereby allowing the slightly smaller deleted amplicons to outcompete the amplification of the wild-type amplicon in the second round of PCR.

Reverse genetics.

WormBook: The Online Review of C. elegans Biology [Internet]. Pasadena (CA): WormBook; 2005-2018.
Fig. 58.7

Detection of SVV DNA in ganglia from monkeys naturally infected with SVV. DNA extracted from pooled cervical (C), thoracic (T), lumbar (L), and sacral (S) ganglia, lung and liver from two monkeys (natural-1 and -2) exposed to an intratracheally infected monkey (intratracheal) was analyzed by nested PCR using primers and probes specific for SVV ORF 63. DNA was omitted in one of the reactions (No DNA). DNA from uninfected (BSC-1 DNA) and SVV-infected BSC-1 cells in culture (SVV-BSC-1 DNA) was used as a negative and positive control, respectively. (Modified and reprinted with permission of J. Virol.)

Simian varicella virus.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge: Cambridge University Press; 2007.