The activator function of Gli2 is required for hair follicle development and is Shh-dependent. Hematoxylin-eosin staining of dorsal skin sections from E18.5 wild type (a), Gli2-/- (b), Shh-/- (c), K5Cre; Z/APGli2; Gli2-/- (m), K5Cre; Z/APGli2; Shh-/- (n), K5Cre; Z/AP ΔNGli2; Gli2-/- (u), and K5Cre; Z/AP ΔNGli2; Shh-/- (v) mice. In situ hybridization analysis of Shh target gene expression, Ptc1 (d, g, j, o, r, w, and z) and Gli1 (e, h, k, p, s, x, and aa). Staining of BrdU (green) and K14 (red) in E18.5 wild type (f ), Gli2-/- (i), Shh-/-(l), K5Cre; Z/APGli2; Gli2-/- (q), K5Cre; Z/APGli2; Shh-/- (t), K5Cre; Z/AP ΔNGli2; Gli2-/- (y), and K5Cre; Z/AP ΔNGli2; Shh-/- (bb) skin. Scale bars: 50μm. Reprinted with permission from: Mill P, Mo R, Fu H et al. Genes Dev 2003; 17(2):282-294. ©2003 CSHL Press Co.