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Total RNA was isolated using TRIZOL method from the leaf tissues collected after 24 hrs of inoculation with early blight pathogen (Alternaria solani). cDNA was prepared using SuperScript Plasmid System for cDNA Synthesis with Gateway (Invitrogen) cloned in pCMV.SPORT6 cloning vector, and transformed in XL1 Blue strain of E.coli.
Nucleotide
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