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The library was constructed by Claire Murphy and Dr. James McCarter at Washington University, St. Louis. The cDNA was made by using Dynabead oligo-dT priming (Dynal). PCR based library using a modified protocol from the SMART PCR cDNA Synthesis Kit from Clontech. Directionally cloned into the UDG sites of pAMP1. Adult nematodes were collected from infected dogs by Dr. Prema Arasu of North Carolina State University, Raleigh, NC (Prema_Arasu@ncsu.edu) and RNA was prepared by Merry McLaird of Divergence Inc, St. Louis, MO (mclaird@divergence.com).
Nucleotide
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