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Interaction-specific EST library

Identifiers
BioSample: SAMN00164395; EST: LIBEST_009892
Organism
Zea mays/Colletotrichum graminicola mixed EST library
cellular organisms; Eukaryota; unclassified eukaryotes; mixed EST libraries
Attributes
strainCgM2
cultivarMutin
tissueInfected leaves
development stage48 to 72 hours post infection
lab hostE. colli Top 10
vectorpCR 4-TOPO (Topo TA cloning kit, Invitrogen)
v_typeplasmid
Description

RNA was isolated from plants 48 and 72 h post infection with Colletotrichum graminicola. After purification of mRNA first strand cDNA synthesis was performed, and infection-specific cDNAs were obtained after subtractive hybridization with photobiotinylated mRNA from vegetative hyphae and healthy maize leaves. The infection-specific cDNAs were subjected to second strand cDNA synthesis, and these cDNAs, representing putative differentially ESTs, were cloned into pCR 4-TOPO.

Submission
Institut fur Pflanzenzuchtung und Pflanzenschutz, Holger B. Deising; 2001-11-01
Accession:
SAMN00164395
ID:
164395

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