4T1, mouse cell line STR profile from ATCC - BioSample - NCBI

Warning: The NCBI web site requires JavaScript to function. more...

Result Filters

4T1, mouse cell line STR profile from ATCC

Identifiers: BioSample: SAMN11397647; Sample name: 4T1

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Package: Model organism or animal; version 1.0

Attributes

cell line	4T1
culture collection	ATCC: CRL-2539
strain	BALB/cfC3H
age	unknown
sex	female
morphology	epithelial-like
tissue	Breast
repository	American Type Culture Collection (ATCC)
disease	Mammary tumor (stage IV)
cell line name alias	4T1-A (Cellosaursus)
date established	Miller, 1983
about cells	tumorigenic, forms tumors and metastasizes in BALB/c mice; serves as a model for stage IV of breast cancer; adherent; thioguanine-resistant variant derived from 410.4 cells without mutagen treatment
mouse cell line STR profile	yes
mouse cell line STR profile status	NIST verified

Description: Sex information derived from Cellosaurus

Links: American Type Culture Collection
ATCC CRL-2539

BioProject: PRJNA539973 Mus musculus
Retrieve all samples from this project

Submission: NIST, Jamie Almeida; 2019-04-11

STR profile
STR 18-3	18,19
STR 4-2	21.3
STR 6-7	12
STR 19-2	13
STR 1-2	17
STR 7-1	25.2
STR 1-1	15,16
STR 3-2	14,15
STR 8-1	13
STR 2-1	16,17
STR 15-3	22.3
STR 6-4	18
STR 11-2	18,19,20
STR 17-2	15
STR 12-1	16
STR 5-5	14
STR X-1	25
STR 13-1	16.2

STR analysis
kit	Type-IT Microsatellite PCR kit (Qiagen) using 18 Mouse STR Markers
DNA source	Frozen vial of cells
DNA isolation method	Quick-DNA Miniprep (Zymo Research, Irvine, CA, USA)
method of DNA quantification	Quant-iT Qubit dsDNA HS Assay Kit (Invitrogen) and fluorescence measured using the Qubit 3 Fluorometer (Invitrogen).
amount of DNA used	2 ng DNA

Accession:: SAMN11397647
ID:: 11397647

Supplemental Content

Related information

BioProject
BioProject links
BioCollections
BioCollections
Taxonomy
Links to Taxonomy

Search details

Recent activity

Clear Turn Off Turn On

txid10090[orgn] AND "culture-collection ATCC CRL-2539"[All Fields... (1)
txid10090[orgn] AND "culture-collection ATCC CRL-2539"[All Fields]
Search

BioSample
4T1, mouse cell line STR profile from ATCC
4T1, mouse cell line STR profile from ATCC

biosample

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

LinkOut to external resources

4T1 (CVCL_0125) [Cellosaurus - a cell line kno...]
4T1 (CVCL_0125)
Cellosaurus - a cell line knowledge resource

You are here: NCBI > DNA & RNA > BioSample