E-MTAB-434:ZF_2cells - BioSample - NCBI

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E-MTAB-434:ZF_2cells

Identifiers: BioSample: SAMEA898400; SRA: ERS017427

Organism: Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio

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description	Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at -70 C Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions. Pellets were resuspended RNase-free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND-1000 Spectrophotometer (Axon Instruments). Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 250 to 300 bp.
development stage	embryo
sample name	ERS017427
sex	mixed
strain	Tuebingen
Alias	E-MTAB-434:ZF_2cells
Broker name	ArrayExpress
INSDC center alias	SC
INSDC center name	Wellcome Sanger Institute
INSDC first public	2011-03-10T17:55:05Z
INSDC last update	2018-03-08T15:25:14Z
INSDC status	public
SRA accession	ERS017427
Title	ZF_2cells

BioProject: PRJEB2333 Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer
Retrieve all samples from this project

Submission: EBI; 2011-03-17

Accession:: SAMEA898400
ID:: 222422

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E-MTAB-434:ZF_2cells
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