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ROMEO2015.016.DNA_2015-07-14_Miss_A_summer_river_water

Identifiers
BioSample: SAMN15506109; Sample name: ROMEO2015.016.DNA; SRA: SRS6986096
Organism
freshwater metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes
Package
Metagenome or environmental; version 1.0
Attributes
isolation sourceriver water
collection date2015-07-14
geographic locationUSA: Mississippi River at Belle Chasse, LA
latitude and longitude29.857150 N 89.977850 W
sample collection device or methodCollected into a 10L carboy dipped below the surface near the center of the river channel
sample material processingMaterial pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPureā„¢ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
sample size1.5 L
isolateMiss_A_summer
replicatereplicate A
Description

USGS 07374525 Mississippi River at Belle Chasse, LA

BioProject
PRJNA645466
Retrieve all samples from this project

Submission
Oregon State University, Byron Crump; 2020-07-10
Accession:
SAMN15506109
ID:
15506109

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