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BioSample

National Center for Biotechnology Information

Organism: Xenopus laevis (African clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus

Attributes

tissue	neurula
development stage	stage 19-23
lab host	DH10B (phage-resistant)
vector	pCS107 (custom)
v_type	plasmid (ampicillin resistant, 200 ug/ml)
re_1	NotI
re_2	SalI

Description: cDNA made by oligo-dT priming. Library constructed by Dr. Francesca Mariani in the laboratory of R. Harland, Ph.D. (University of California, Berkeley). References: XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue. Mariani, FV. Harland, RM., Development. 1998 Dec;125(24):5019-31. PMID: 9811586; UI: 99030283; Use of large-scale expression cloning screens in the xenopus laevis tadpole to identify gene function. Grammer TC. Liu KJ. Mariani FV. Harland RM,. Dev Biol. 2000 Dec 15;228(2):197-210. PMID: 11112324; UI: 20564075; Note: This is a Xenopus Gene Collection (XGC) library.