BioSample

F2, F3, and F4 lines of Aquilegia formosa X A. pubescens were grown from seed in greenhouses at UC Santa Barbara. From these plants three sets of tissue were collected: 1) Small flower buds (<10 mm) and very young inflorescences (71 & 29% by weight respectively), 2) Medium (7-20 mm) and large (at or near anthesis) flower buds (65 & 35% by weight respectively) and 3) Shoot apical meristems. A fourth set of tissue was collected from plants of A. formosa. These plants were grown from seed in sand and at approximately 1 month root tissue and leaf tissue of various developmental stages were collected (84 & 16% by weight respectively). Total RNA was extracted from each set of tissue and pooled in the following proportions: 1.5X from sets 1 & 2, 1X from sets 3 & 4. From the pooled total RNA, mRNA was extracted and enriched for full-length messages and then normalized with proprietary methods by Invitrogen.