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NIH_XGC_tropMet6

Identifiers
BioSample: SAMN00166602; EST: LIBEST_016866
Organism
Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana
Attributes
tissuewhole embryo
development stageMetamorphic (st.62)
lab hostElectroMAX DH10B
vectorpCMVsport6
re_1SalI
re_2NotI
Description

This library was made from dT primed cDNA and cloned into Invitrogen pCMVsport6 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCMVsport6 vector.

Submission
DOE Joint Genome Institute, Lindquist,E.A., Richardson,P.; 2005-01-04
Accession:
SAMN00166602
ID:
166602

Nucleotide

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