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BioSample

National Center for Biotechnology Information

Organism: Xenopus laevis (African clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus

Attributes

tissue	whole embryo, hyperdorsalized by LiCl treatment
development stage	stage 13
lab host	XL1-Blue, F+
vector	pBSRN3 (modified pBluescript KS+, see description below)
v_type	plasmid (ampicillin resistant)
re_1	NotI
re_2	EcoRI

Description: cDNA made by oligo-dT priming. Directionally cloned into EcoRI/NotI sites using the following 5' adaptor: gaattccccggg and 3' adaptor: agagaactagtgtcgacgcggccgcttttttttttttttttttt. Vector designed for mRNA injections (reference Lemaire et al. (1995) 81:85-94). Average insert size 1.6kb. Primary library, non-amplified. Library constructed by N. Pollet, PhD REF: Glinka, et al. (1996) Combinatorial signaling by Xwnt-11 and Xnr3 in the organizer epithelium. Mech. Dev. 60:221-231. Note: This is a Xenopus Gene Collection (XGC) library.