NCI_CGAP_Sub5 is a subtracted library derived from NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub4 was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991,1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759,1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351). (10% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the driver population), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the driver population), plus a pool of 5,472 clones from NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.