BioSample

Rice is one of two most popular grains in the world. Half of the world population especially those inhabiting highly populated areas of the humid tropics and subtropics, rely on rice as their primary source of carbohydrate. Monocotyledonous rice is a diploid plant (2n=24) with a haploid genome equivalent of 431 Mbp (Arumuganathan and Earle, 1991). The relatively small genome of rice, three times larger than that of Arabidopsis, makes it suitable for genomic studies. In order to facilitate positional cloning, physical mapping and genome sequencing of rice, we have constructed a BAC library from Oryza sativa, Nipponbare variety. The library contains 36,864 clones with an average insert size of 128.5 Kb providing 10.9 haploid genome equivalents. The deep coverage allows the isolation a particular sequence with a probability of 99.9 %. Two high density filters, each containing 18,432 clones (doubly spotted), represent the whole library for colony screening.