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NIH_XGC_tropInt1

Identifiers
BioSample: SAMN00166598; EST: LIBEST_016862
Organism
Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana
Attributes
strainN6 (Nigerian 6th generation inbred)
tissueIntestine
development stageAdult
lab hostElectroMAX DH10B T1 Phage Resistant cells
vectorpCS107
re_1EcoRI
re_2XhoI
Description

The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Submission
DOE Joint Genome Institute, Lindquist,E.A., Richardson,P.; 2005-01-04
Accession:
SAMN00166598
ID:
166598

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