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BioSample

National Center for Biotechnology Information

Organism: Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana

Attributes

strain	N6 (Nigerian 6th generation inbred)
tissue	whole embryo
development stage	Neurula (st. 14-18)
lab host	TOP10 E. coli
vector	pCS22+
re_1	PstI
re_2	XhoI

Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (V(T)20CTCGAGAGAGAGAGAG). After ligation of PstI adapters and by XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).