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The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (V(T)20CTCGAGAGAGAGAGAG). After ligation of PstI adapters and by XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
Nucleotide
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