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Gastrula library constructed by Russell B. Fletcher in R. Harland's lab using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). SalI (5' end) -NotI (3' end) cDNA was inserted into vector pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html).
Nucleotide
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