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At stage 8 ectoderm of Xenopus laevis embryos were cut and disaggregated. The cells were treated with activin at a concentration to induce endodermal tissue. Afterwards the reaggregated cells were cultured until control sibling embryos reaching stage 12/13. An expression library was constructed from such induced endodermal tissue using ZAP Express Kits.
Nucleotide
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