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Constructed by Sara Melville, University of Cambridge, UK and Nancy Shepherd, Dupont Merck, Wilmington, DE. Genomic DNA was isolated from Trypanosoma brucei (stock TREU927/4) and partially digested with Sau 3AI. DNA fragments were cloned into the Bam H1 site of pAD10SacBII vector (Genbank accession U09128). The average insert size is 65 Kb. Coverage: approx 4.4 X the haploid non-minichromosomal genome
Nucleotide
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