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Normalized Anopheles Head (NAH) Library

Identifiers
BioSample: SAMN00173642; EST: LIBEST_014203
Organism
Anopheles gambiae (African malaria mosquito)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae; Anophelinae; Anopheles; Cellia; Pyretophorus; gambiae species complex
Attributes
lab hostE. coli DH10B
vectorpT7T3D-PacI
re_1EcoR1 (5'end)
re_2Not1 (3'end)
Description

a directionally cloned and normalized, oligo-T primed cDNA library constructed from strain 4arr adult mosquito heads. Equal numbers of sugar fed males, sugar fed females and 6, 24 and 48 hr post blood meal females were used: Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery, Genome Research 6, 791-806. ESTs sequenced from the M13 reverse priming site reading from the 5' ends of the cDNAs are indicated by 'R' in the clone name. ESTs sequenced from the M13 forward priming site reading from the 3' ends of the cDNAs are indicated by 'F' in the clone name.

Submission
University of Notre Dame, Frank H. Collins; 2003-08-07
Accession:
SAMN00173642
ID:
173642

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