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4ug of mRNA from Haementeria depressa salivary complexes were primed with oligo- (dT) and reverse transcribed to cDNA using Superscript Plasmid System for cDNA Synthesis and Cloning (Invitrogen). The cDNAs were selected by size (400-800 pb and up 800 pb) in agarose gel electrophoresis, linked to Eco RI adapters and directionally cloned in pGEM11Zf+ vector (Promega). ESTs were generated from random clones and grouped in unique sequences. The putative identification of each EST or cluster was obtained through Blast searches (e-value < e-05).
Nucleotide
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