NCI_CGAP_HN5

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Result Filters

Organism: Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo

Attributes

tissue	normal gingiva (cell line from primary keratinocytes)
lab host	SOLR (kanamycin resistant)
vector	Bluescript SK-
v_type	phagemid (ampicillin resistant)
re_1	EcoRI
re_2	XhoI

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' AATTCGGCACGAG 3' 3' GCCGTGCTC 5' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' EcoRI site appears to have been lost in a fraction of the clones. Library constructed by Stratagene; available through Mary May, PhD (Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH; mmay@yoda.nidr.nih.gov).