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Xenopus tropicalis xthr plasmid library

Identifiers
BioSample: SAMN00166584; EST: LIBEST_015540
Organism
Xenopus tropicalis (tropical clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana
Attributes
strainIvory Coast
tissuepool of heads and retinas from tailbud stages 25-35
development stagestage 25-35
lab hostE.coli DH10B
vectorpCMVSPORT6_xthr
v_typePlasmid
re_1SfiI
re_2SfiI
Description

Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).

Submission
CNRS UMR 8080, N Pollet; 2004-03-30
Accession:
SAMN00166584
ID:
166584

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