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These sequences resulted from single pass sequencing of PCR products from Compugen targeted mRNA sequencing project. PCR primers and nested primers were designed to join two neighboring expressed sequence contigs based on Compugen LEADS expressed sequence cluster and assembly platform. Reverse transcriptase PCR and nested PCR reactions were performed by Protedyne Inc. (Windsor, CT 06095, USA) on normal human heart, brain, lung, liver, placenta, and testis poly mRNA preparations from Origen (Rockville, MD 20850, USA) with reagents from Invitrogen (Carlsbad, California 92008). PCR products were sequenced by Qiagen (Valencia, CA 91355).
Nucleotide
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