Meloidogyne incognita parasitic J2 J3 SL1 TOPO v1

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Result Filters

Organism: Meloidogyne incognita (southern root-knot nematode)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Tylenchina; Tylenchomorpha; Tylenchoidea; Meloidogynidae; Meloidogyninae; Meloidogyne; Meloidogyne incognita group

Attributes

development stage	parasitic J2/J3
lab host	DH10B
vector	pCRII-TOPO(Invitrogen)
v_type	plasmid (Kanamycin and Ampicillin resistant)
re_1	EcoRI
re_2	EcoRI

Description: The library was constructed by Irina Ronko and Dr. Makedonka Dautova (mdautova@watson.wustl.edu) at Washington University, St.Louis. Oligo (dT)-SL1 based library. Meloidogyne incognita J2/J3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5'end and oligo (dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Roots infected with Meloidogyne incognita were provided by Dr.Barry Shortt at Divergence,Inc. (shortt@divergence.com).