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SSR enriched library was constructed using genomic DNA isolated from Vicia faba L. variety Giza 716. SSR enriched fragments were used for TA cloning using pGEM Easy cloning kit (Promega, Madison,WI USA) following manufacture's recommended protocol. Transformed bacterial clones were sequenced by ABI capillary sequencer 3130xl using T7 and SP6 primers and sequences were screened for SSR motives. Specific primers flanking SSR motives were designed using Bachprimer3.
Nucleotide
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