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mRNA were extracted from field grown plants (Dunkerque, France). Following harvest, roots, leaves and flowers were cut-off and discarded, as were the bottom 10 cm of the stem. The following bottom 15-cm section of the stem was then isolated and the outer fiber-bearing tissues peeled off and immediately frozen in liquid nitrogen. Library was constructed using ZAP-cDNA synthesis kit and ZAP-cDNA Gigapack III cloning kit (Stratagene).
Nucleotide
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