NICHD_XGC_Te1

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Result Filters

Organism: Xenopus laevis (African clawed frog)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus

Attributes

tissue	Normal testis
lab host	DH10B TonA
vector	pDNR-LIB
v_type	plasmid (chloramphenicol resistant, grown at 30C)
re_1	SfiI (directional)
re_2	SfiI (directional)

Description: Non-normalized full-length enriched library 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'(where B = A, C, or G and N = A, C, G, or T). Average insert size 1.2kb (cDNA range 0.4-3.0 kb). 15/15 coloniescontained inserts by PCR.This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).Corp.