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subtracted cochlea cDNA library

Identifiers
BioSample: SAMN00172308; EST: LIBEST_012768
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuecochlea
development stage16-22 week fetus
lab hostTOP10F'
tissueear
vectorpCRII
Description

Subtraction of cochlea cDNA (tester) was performed with the PCR-Select cDNA Subtraction Kit (Clontech). Driver cDNA consisted of a mixture of cDNAs from brain, kidney, liver, heart, skeletal muscle, placenta, stomach (mucosal layer) and psoas muscle. Tester and driver cDNAs were digested by RsaI; adaptors were ligated to the tester. Two rounds of subtractive hybridization were performed, followed by two suppression PCR reactions. Subtracted PCR products were ligated into a T/A vector (Invitrogen).

Submission
Radboud University Nijmegen Medical Centre, Kremer H; 2003-03-20
Accession:
SAMN00172308
ID:
172308

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